Joshua D. Greenlee

[email protected]

7277 Charlotte Pike UNIT 311 – Nashville, TN – 37209

740-851-3853

EDUCATION

Vanderbilt University, Nashville, TN                                    Doctor of Philosophy – PhD, Biomedical Engineering              2017 – Present                                                                  GPA: 3.87

Ohio University, Athens, OH                                                  Bachelor of Science – BS, Chemical Engineering                    2013 – 2017                                                                      Summa Cum Laude                                                                  GPA: 3.98                                                                            

HONORS AND AWARDS

  • National Science Foundation Graduate Research Fellowship Awardee  (2018)
  • 2017-18 Thomas R. Harris Graduate Fellowship (2017)
  • National Science Foundation Graduate Research Fellowship - Honorable Mention (2017)
  • American Chemical Society (ACS) Society of Chemical Industry (SCI) Scholar (2016)
  • Chemical and Biomolecular Engineering Keith Russ Outstanding Junior Award (2016)
  • Appalachian Cohort for Engineering Scholar (2013)
  • Alexander High School Valedictorian  (2013)

 RESEARCH EXPERIENCE

Vanderbilt University, Dr. Michael King, Biomedical Research Laboratory, Nashville, TN                      2017 – Present

Graduate Research Fellow

  • Develop novel drug delivery strategies for the liposomal delivery of anti-cancer moieties to metastatic cancers
  • Investigate TRAIL applications in oxaliplatin-resistant metastatic colorectal cancer
  • Elucidate mechanisms of chemoresistant induced TRAIL sensitivity
  • Investigate and develop orthotopic xenograft models of colorectal cancer in vivo
  • Fabricate in vitro microfluidic devices to model lymphatic metastasis
  • Employ the use of molecular biology techniques such as immunofluorescent staining, flow cytometry, ELISA and qRT-PCR
  • Process metastatic cancer patient blood samples for modeling drug efficacy ex vivo
  • Process patient RNAseq data to determine molecular targets within intrinsic and extrinsic apoptotic pathways
  • Optimize recombinant protein production in E. coli and downstream purification using liquid chromatography

Ohio University, Dr. Doug Goetz, Biomedical Research Laboratory, Athens, OH                                                       2015 –2017

Undergraduate Research Assistant

  • Study the effects of a methimazole derivative in the modulation of specific cytokine expression in cancer cells
  • Investigate and test a new compound in the treatment of ovarian cancer
  • Grow and culture cells for testing
  • Determine drug efficacy and toxicity through tests such as ELISA and qPCR
  • Assist in the publication of graduate student thesis

Ohio University, Institute for Sustainable Energy and the Environment, Athens, OH                                                  2014 –2015

Undergraduate Research Assistant              

  • Design and modify engineering systems for experimentation
  • Write experimental reports and protocols such as SOPs and SERs
  • Assist graduate students on research projects in fracking wastewater and coal repurposing
  • Use analytical instruments such as gas chromatography – mass spectrometry (GC-MS) and inductively coupled plasma (ICP) for sample analysis
  • Train new undergraduate and graduate students on using analytical instruments
  • Perform data analysis on analytical and chemical testing results

 

PUBLICATIONS

  • Hope, J., Greenlee, J. & King, M. Mechanosensitive Ion Channels: TRPV4 and P2X7 in Disseminating Cancer Cells. The Cancer Journal 24, 84–92 (2018).
  • Greenlee J. & King, M. Engineered Fluidic Systems to Understand Lymphatic Cancer Metastasis. Biomicrofluidics (2019). *In press*

 

CONFERENCE PRESENTATIONS

Oral Presentations

  • Greenlee, J., Zhang, Z., Yu, F., Lui, D., King, M. “Trail-Conjugated Liposomes that Kill Colorectal Cancer Metastases in the Lymph Nodes”. Biomedical Engineering Society; October 2018; Atlanta, GA.

 

Posters

  • Greenlee, J., King, M. “TRAILing a Chemoresistant Phenotype: TRAIL Applications as an Adjuvant for Oxaliplatin-Resistant Colorectal Cancer”. Vanderbilt-Ingram Cancer Center 20th Annual Scientific Retreat; May 2019; Nashville, TN. *Awarded 3rdt Place*
  • Greenlee, J., Zhang, Z., Yu, F., Lui, D., King, M. “TRAIL-Conjugated Liposomes to Kill Chemoresistant Cancer Cells in the Lymph Nodes”. Society For Biomaterials; April 2018; Atlanta, GA. *Awarded 2nd Place Immune Engineering SIG*
  • Champa, Z., Greenlee, J., Whalen, D., Goetz, D., Bergmeier, S., McCall, K. “Inhibition of IL-6 and IL-8 Expression in Ovarian Cancer by a Phenylmethimazole Derivative”. Ohio University Student Research Exposition; April 2016; Athens, OH. *Awarded 1st Place in Chemical and Biomolecular Engineering Category*

TEACHING EXPERIENCE

The SyBBURE Searle Undergraduate Research Program, Vanderbilt University, Nashville, TN           2018 – Present

SyBBURE Searle Student Scientific Advisor

  • Incubate and inspire the next generation of innovators through research, design, and community
  • Lead weekly subgroup meetings with undergraduate students to provide insights and advice on research topics
  • Advise students in weekly ThinkTank meetings on invention designs
  • Teach students about topics such as market analysis, commercialization strategy and prototyping

 

Vanderbilt University, Nashville, TN        2017 – 2018

Teaching Assistant

  • TA for two undergraduate biomedical Engineering courses: “Medical Device Innovation” and “Introduction to Biomedical Engineering”
  • Prepare and present lectures on engineering and design related subjects
  • Grade and provide feedback on student assignments

CORPORATE EXPERIENCE

Eastman Chemical Company, Corporate Innovation and Technology, Kingsport, TN                                                        2016

Chemical Engineering Intern

  • Research cellulose ester applications in electronics and printability
  • Compare cellulose ester properties with other polymers
  • Propose new products from polymer property data
  • Assess marketing potential of proposed products
  • Present innovative approaches for new applications of cellulose esters to Eastman marketers
  • Coordinate and lead cross continental meetings with branches in Shanghai and Singapore
  • Work 40 hours per week in the summer

OTHER WORK EXPERIENCE                     

Ohio University, Golf and Tennis Center, Athens, OH              2013 – 2014

Grounds RA                                

  • Maintain the Ohio University Golf Course including greens, fairways and bunkers
  • Responsible for specialized lawn mowing equipment
  • Work 10 hours per week in the Fall, Spring and Summer

Self-Employed, Athens OH                 2009 –2014

Lawn Care and Landscaping                      

  • Mow and landscape lawns in Athens County
  • Demonstrate care for equipment and property 

TECHNICAL SKILLS AND QUALIFICATIONS

  • Extensive course load in chemical and biomolecular engineering
  • Proficient using Microsoft Office, MATLAB, ChemCAD, and R
  • Advanced molecular biology techniques including Flow cytometry, FACS, siRNA knockdown, lentiviral transfection, ELISA and RT-PCR
  • Device microfabrication techniques such as photolithography, chemical and ion etching
  • Experience designing and conducting research as an undergraduate and PhD student
  • Experience teaching, tutoring and mentoring undergraduate students in academics and research
  • Extensive experience public speaking at national conferences and in a classroom setting

 

ACTIVITIES, ORGANIZATION AN VOLUNTEER WORK

  • Vanderbilt Biomedical Engineering GSA Social Chair (2017 – 2019)
  • Glencliff High School Volunteer Mentor (2017 – 2018)
  • Tau Beta Pi National Engineering Honor Society – Activities Chair Officer  (2015 – 2017)
  • Russ College of Engineering Ambassador  (2016 – 2017)
  • WERC Environmental National Design Competition (2016 – 2017)
  • Biomedical Engineering Society (2016 – present)
  • Athens County Young Life Leader  (2013 – 2017)
  • National Society of Collegiate Scholars (2013 – 2017)
  • Landscaping Volunteer at Albany Baptist Church       (2009 – 2017)
  • Appalachian Cohort for Engineering  (2013 – 2017)
  • American Institute of Chemical Engineers (2014 – present)