Joshua D. Greenlee
[email protected]
7277 Charlotte Pike UNIT 311 – Nashville, TN – 37209
740-851-3853
EDUCATION
Vanderbilt University, Nashville, TN Doctor of Philosophy – PhD, Biomedical Engineering 2017 – Present GPA: 3.87
Ohio University, Athens, OH Bachelor of Science – BS, Chemical Engineering 2013 – 2017 Summa Cum Laude GPA: 3.98
HONORS AND AWARDS
- National Science Foundation Graduate Research Fellowship Awardee (2018)
- 2017-18 Thomas R. Harris Graduate Fellowship (2017)
- National Science Foundation Graduate Research Fellowship - Honorable Mention (2017)
- American Chemical Society (ACS) Society of Chemical Industry (SCI) Scholar (2016)
- Chemical and Biomolecular Engineering Keith Russ Outstanding Junior Award (2016)
- Appalachian Cohort for Engineering Scholar (2013)
- Alexander High School Valedictorian (2013)
RESEARCH EXPERIENCE
Vanderbilt University, Dr. Michael King, Biomedical Research Laboratory, Nashville, TN 2017 – Present
Graduate Research Fellow
- Develop novel drug delivery strategies for the liposomal delivery of anti-cancer moieties to metastatic cancers
- Investigate TRAIL applications in oxaliplatin-resistant metastatic colorectal cancer
- Elucidate mechanisms of chemoresistant induced TRAIL sensitivity
- Investigate and develop orthotopic xenograft models of colorectal cancer in vivo
- Fabricate in vitro microfluidic devices to model lymphatic metastasis
- Employ the use of molecular biology techniques such as immunofluorescent staining, flow cytometry, ELISA and qRT-PCR
- Process metastatic cancer patient blood samples for modeling drug efficacy ex vivo
- Process patient RNAseq data to determine molecular targets within intrinsic and extrinsic apoptotic pathways
- Optimize recombinant protein production in E. coli and downstream purification using liquid chromatography
Ohio University, Dr. Doug Goetz, Biomedical Research Laboratory, Athens, OH 2015 –2017
Undergraduate Research Assistant
- Study the effects of a methimazole derivative in the modulation of specific cytokine expression in cancer cells
- Investigate and test a new compound in the treatment of ovarian cancer
- Grow and culture cells for testing
- Determine drug efficacy and toxicity through tests such as ELISA and qPCR
- Assist in the publication of graduate student thesis
Ohio University, Institute for Sustainable Energy and the Environment, Athens, OH 2014 –2015
Undergraduate Research Assistant
- Design and modify engineering systems for experimentation
- Write experimental reports and protocols such as SOPs and SERs
- Assist graduate students on research projects in fracking wastewater and coal repurposing
- Use analytical instruments such as gas chromatography – mass spectrometry (GC-MS) and inductively coupled plasma (ICP) for sample analysis
- Train new undergraduate and graduate students on using analytical instruments
- Perform data analysis on analytical and chemical testing results
PUBLICATIONS
- Hope, J., Greenlee, J. & King, M. Mechanosensitive Ion Channels: TRPV4 and P2X7 in Disseminating Cancer Cells. The Cancer Journal 24, 84–92 (2018).
- Greenlee J. & King, M. Engineered Fluidic Systems to Understand Lymphatic Cancer Metastasis. Biomicrofluidics (2019). *In press*
CONFERENCE PRESENTATIONS
Oral Presentations
- Greenlee, J., Zhang, Z., Yu, F., Lui, D., King, M. “Trail-Conjugated Liposomes that Kill Colorectal Cancer Metastases in the Lymph Nodes”. Biomedical Engineering Society; October 2018; Atlanta, GA.
Posters
- Greenlee, J., King, M. “TRAILing a Chemoresistant Phenotype: TRAIL Applications as an Adjuvant for Oxaliplatin-Resistant Colorectal Cancer”. Vanderbilt-Ingram Cancer Center 20th Annual Scientific Retreat; May 2019; Nashville, TN. *Awarded 3rdt Place*
- Greenlee, J., Zhang, Z., Yu, F., Lui, D., King, M. “TRAIL-Conjugated Liposomes to Kill Chemoresistant Cancer Cells in the Lymph Nodes”. Society For Biomaterials; April 2018; Atlanta, GA. *Awarded 2nd Place Immune Engineering SIG*
- Champa, Z., Greenlee, J., Whalen, D., Goetz, D., Bergmeier, S., McCall, K. “Inhibition of IL-6 and IL-8 Expression in Ovarian Cancer by a Phenylmethimazole Derivative”. Ohio University Student Research Exposition; April 2016; Athens, OH. *Awarded 1st Place in Chemical and Biomolecular Engineering Category*
TEACHING EXPERIENCE
The SyBBURE Searle Undergraduate Research Program, Vanderbilt University, Nashville, TN 2018 – Present
SyBBURE Searle Student Scientific Advisor
- Incubate and inspire the next generation of innovators through research, design, and community
- Lead weekly subgroup meetings with undergraduate students to provide insights and advice on research topics
- Advise students in weekly ThinkTank meetings on invention designs
- Teach students about topics such as market analysis, commercialization strategy and prototyping
Vanderbilt University, Nashville, TN 2017 – 2018
Teaching Assistant
- TA for two undergraduate biomedical Engineering courses: “Medical Device Innovation” and “Introduction to Biomedical Engineering”
- Prepare and present lectures on engineering and design related subjects
- Grade and provide feedback on student assignments
CORPORATE EXPERIENCE
Eastman Chemical Company, Corporate Innovation and Technology, Kingsport, TN 2016
Chemical Engineering Intern
- Research cellulose ester applications in electronics and printability
- Compare cellulose ester properties with other polymers
- Propose new products from polymer property data
- Assess marketing potential of proposed products
- Present innovative approaches for new applications of cellulose esters to Eastman marketers
- Coordinate and lead cross continental meetings with branches in Shanghai and Singapore
- Work 40 hours per week in the summer
OTHER WORK EXPERIENCE
Ohio University, Golf and Tennis Center, Athens, OH 2013 – 2014
Grounds RA
- Maintain the Ohio University Golf Course including greens, fairways and bunkers
- Responsible for specialized lawn mowing equipment
- Work 10 hours per week in the Fall, Spring and Summer
Self-Employed, Athens OH 2009 –2014
Lawn Care and Landscaping
- Mow and landscape lawns in Athens County
- Demonstrate care for equipment and property
TECHNICAL SKILLS AND QUALIFICATIONS
- Extensive course load in chemical and biomolecular engineering
- Proficient using Microsoft Office, MATLAB, ChemCAD, and R
- Advanced molecular biology techniques including Flow cytometry, FACS, siRNA knockdown, lentiviral transfection, ELISA and RT-PCR
- Device microfabrication techniques such as photolithography, chemical and ion etching
- Experience designing and conducting research as an undergraduate and PhD student
- Experience teaching, tutoring and mentoring undergraduate students in academics and research
- Extensive experience public speaking at national conferences and in a classroom setting
ACTIVITIES, ORGANIZATION AN VOLUNTEER WORK
- Vanderbilt Biomedical Engineering GSA Social Chair (2017 – 2019)
- Glencliff High School Volunteer Mentor (2017 – 2018)
- Tau Beta Pi National Engineering Honor Society – Activities Chair Officer (2015 – 2017)
- Russ College of Engineering Ambassador (2016 – 2017)
- WERC Environmental National Design Competition (2016 – 2017)
- Biomedical Engineering Society (2016 – present)
- Athens County Young Life Leader (2013 – 2017)
- National Society of Collegiate Scholars (2013 – 2017)
- Landscaping Volunteer at Albany Baptist Church (2009 – 2017)
- Appalachian Cohort for Engineering (2013 – 2017)
- American Institute of Chemical Engineers (2014 – present)